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KMID : 1138520130160030030
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Journal of Pharmacopuncture
2013 Volume.16 No. 3 p.30 ~ p.38
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Differential Expression of Gene Profiles in MRGX-treated Lung Cancer
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Kwon Yong-Kyun
Lee Seung-Yeul
Kang Hwan-Soo
Sung Jung-Suk
Cho Chong-Kwan
Yoo Hwa-Seung
Shin Seung-Jin
Choi Jong-Soon
Lee Yeon-Weol
Jang Ik-Soon
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Abstract
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Objectives: Modified regular ginseng extract (MRGX) has stronger anti-cancer activity-possessing gensenoside profiles.
Methods: To investigate changes in gene expression in the MRGX-treated lung cancer cells (A549), we examined genomic data with cDNA microarray results. After completing the gene-ontology-based analysis, we grouped the genes into up-and down-regulated profiles and into ontology-related regulated genes and proteins through their interaction network.
Results: One hundred nine proteins that were up- and down-regulated by MRGX were queried by using IPA. IL8, MMP7 and PLAUR and were found to play a major role in the anti-cancer activity in MRGX-treated lung cancer cells. These results were validated using a Western blot analysis and a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis.
Conclusions: Most MRGX-responsive genes are up-regulated transiently in A549 cells, but down-regulated in a sustained manner in lung cancer cells.
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KEYWORD
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gene ontology, lung cancer, microarray, modified regular ginseng extract (MRGX)
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